resource source identifier critical Search Results


resource source identifier qiaprep spin miniprep kit qiagen  (Qiagen)
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Qiagen resource source identifier qiaprep spin miniprep kit qiagen
Resource Source Identifier Qiaprep Spin Miniprep Kit Qiagen, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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resource source identifier microcentrifuge eppendorf 5424 centrifuge  (Eppendorf AG)
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Eppendorf AG resource source identifier microcentrifuge eppendorf 5424 centrifuge
Resource Source Identifier Microcentrifuge Eppendorf 5424 Centrifuge, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reagent or resource source identifier other neurostar robot stereotax  (Neurostar GmbH)
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Neurostar GmbH reagent or resource source identifier other neurostar robot stereotax
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kod dna polymerase  (Merck & Co)
90
Merck & Co kod dna polymerase
Kod Dna Polymerase, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reagent or resource source identifier sciscan  (Scientifica)
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Scientifica reagent or resource source identifier sciscan
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mouse monoclonal anti-l1hs-orf1p  (Merck & Co)
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Mouse Monoclonal Anti L1hs Orf1p, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tb cryptate-conjugated mouse monoclonal anti-flag antibody  (Cisbio Bioassays)
90
Cisbio Bioassays tb cryptate-conjugated mouse monoclonal anti-flag antibody
(A) Schematic illustration of the design of the TR-FRET assay for monitoring the SMAD4/SMAD3 PPI to discover small-molecule PPI inducers. <t>Anti-Flag-Tb</t> coupled with Flag-SMAD3 serves as the TR-FRET donor and anti-His-D2 coupled with His-SMAD4R361H serves as the acceptor. At the basal level, R361H impairs SMAD4 interaction with SMAD3, yielding low TR-FRET signal. Upon treatment with a PPI inducer, the induced SMAD3/SMAD4R361H complex formation brings two fluorophores into close proximity (<10 nm), generating a high TR-FRET signal.
Tb Cryptate Conjugated Mouse Monoclonal Anti Flag Antibody, supplied by Cisbio Bioassays, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti-h3k27me3  (Merck & Co)
90
Merck & Co rabbit anti-h3k27me3
(A) Schematic illustration of the design of the TR-FRET assay for monitoring the SMAD4/SMAD3 PPI to discover small-molecule PPI inducers. <t>Anti-Flag-Tb</t> coupled with Flag-SMAD3 serves as the TR-FRET donor and anti-His-D2 coupled with His-SMAD4R361H serves as the acceptor. At the basal level, R361H impairs SMAD4 interaction with SMAD3, yielding low TR-FRET signal. Upon treatment with a PPI inducer, the induced SMAD3/SMAD4R361H complex formation brings two fluorophores into close proximity (<10 nm), generating a high TR-FRET signal.
Rabbit Anti H3k27me3, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reagent or resource source identifier a05  (AK Scientific)
90
AK Scientific reagent or resource source identifier a05
(A) Schematic illustration of the design of the TR-FRET assay for monitoring the SMAD4/SMAD3 PPI to discover small-molecule PPI inducers. <t>Anti-Flag-Tb</t> coupled with Flag-SMAD3 serves as the TR-FRET donor and anti-His-D2 coupled with His-SMAD4R361H serves as the acceptor. At the basal level, R361H impairs SMAD4 interaction with SMAD3, yielding low TR-FRET signal. Upon treatment with a PPI inducer, the induced SMAD3/SMAD4R361H complex formation brings two fluorophores into close proximity (<10 nm), generating a high TR-FRET signal.
Reagent Or Resource Source Identifier A05, supplied by AK Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trna (bacterial, mre600  (Merck & Co)
90
Merck & Co trna (bacterial, mre600
(A) Schematic illustration of the design of the TR-FRET assay for monitoring the SMAD4/SMAD3 PPI to discover small-molecule PPI inducers. <t>Anti-Flag-Tb</t> coupled with Flag-SMAD3 serves as the TR-FRET donor and anti-His-D2 coupled with His-SMAD4R361H serves as the acceptor. At the basal level, R361H impairs SMAD4 interaction with SMAD3, yielding low TR-FRET signal. Upon treatment with a PPI inducer, the induced SMAD3/SMAD4R361H complex formation brings two fluorophores into close proximity (<10 nm), generating a high TR-FRET signal.
Trna (Bacterial, Mre600, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ethylenediaminetetraacetic acid  (Merck & Co)
90
Merck & Co ethylenediaminetetraacetic acid
(A) Schematic illustration of the design of the TR-FRET assay for monitoring the SMAD4/SMAD3 PPI to discover small-molecule PPI inducers. <t>Anti-Flag-Tb</t> coupled with Flag-SMAD3 serves as the TR-FRET donor and anti-His-D2 coupled with His-SMAD4R361H serves as the acceptor. At the basal level, R361H impairs SMAD4 interaction with SMAD3, yielding low TR-FRET signal. Upon treatment with a PPI inducer, the induced SMAD3/SMAD4R361H complex formation brings two fluorophores into close proximity (<10 nm), generating a high TR-FRET signal.
Ethylenediaminetetraacetic Acid, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reagent or resource source identifier anymaze  (Stoelting inc)
90
Stoelting inc reagent or resource source identifier anymaze
(A) Schematic illustration of the design of the TR-FRET assay for monitoring the SMAD4/SMAD3 PPI to discover small-molecule PPI inducers. <t>Anti-Flag-Tb</t> coupled with Flag-SMAD3 serves as the TR-FRET donor and anti-His-D2 coupled with His-SMAD4R361H serves as the acceptor. At the basal level, R361H impairs SMAD4 interaction with SMAD3, yielding low TR-FRET signal. Upon treatment with a PPI inducer, the induced SMAD3/SMAD4R361H complex formation brings two fluorophores into close proximity (<10 nm), generating a high TR-FRET signal.
Reagent Or Resource Source Identifier Anymaze, supplied by Stoelting inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) Schematic illustration of the design of the TR-FRET assay for monitoring the SMAD4/SMAD3 PPI to discover small-molecule PPI inducers. Anti-Flag-Tb coupled with Flag-SMAD3 serves as the TR-FRET donor and anti-His-D2 coupled with His-SMAD4R361H serves as the acceptor. At the basal level, R361H impairs SMAD4 interaction with SMAD3, yielding low TR-FRET signal. Upon treatment with a PPI inducer, the induced SMAD3/SMAD4R361H complex formation brings two fluorophores into close proximity (<10 nm), generating a high TR-FRET signal.

Journal: Cell chemical biology

Article Title: Hypomorph mutation-directed small-molecule protein-protein interaction inducers to restore mutant SMAD4-suppressed TGF-β signaling

doi: 10.1016/j.chembiol.2020.11.010

Figure Lengend Snippet: (A) Schematic illustration of the design of the TR-FRET assay for monitoring the SMAD4/SMAD3 PPI to discover small-molecule PPI inducers. Anti-Flag-Tb coupled with Flag-SMAD3 serves as the TR-FRET donor and anti-His-D2 coupled with His-SMAD4R361H serves as the acceptor. At the basal level, R361H impairs SMAD4 interaction with SMAD3, yielding low TR-FRET signal. Upon treatment with a PPI inducer, the induced SMAD3/SMAD4R361H complex formation brings two fluorophores into close proximity (<10 nm), generating a high TR-FRET signal.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Tb cryptate-conjugated mouse monoclonal anti-Flag antibody Cisbio Bioassays Cat# 61FG2TLB D2-conjugated anti-HIS antibody Cisbio Bioassays Cat# 61HISDLF Mouse monoclonal anti-FLAG-HRP antibody Sigma-Aldrich Cat# A8592; RRID: AB_439702 Rabbit polyclonal anti-GST-HRP antibody Sigma-Aldrich Cat# A7340; RRID: AB_258340 Mouse monoclonal anti-β-Actin antibody Sigma-Aldrich Cat# A5441; RRID: AB_476744 Rabbit monoclonal anti-p21 antibody Cell signaling Cat# 2947S; RRID: AB_823586 Mouse monoclonal anti-p15 antibody Santa Cruz Cat# sc-271791; RRID: AB_10709436 SMAD4 (D3M6U) Rabbit mAb Cell signaling Cat# 38454; RRID: AB_2728776 SMAD3 mouse monoclonal Antibody (38-Q) Santa Cruz Cat# sc-101154; RRID: AB_1129525 Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) Cell signaling Cat# 93702; RRID: AB_2800208 Peroxidase AffiniPure Goat Anti-Rabbit IgG(H+L) secondary antibody Jackson ImmunoResearch Cat# 111–035-003 Peroxidase AffiniPure Goat Anti-Mouse IgG(H+L) secondary antibody Jackson ImmunoResearch Cat# 115–035-003 Chemicals, Peptides, and Recombinant Proteins Recombinant Human TGF-beta 1 Protein R&D Systems Cat# 240-B Ro-31-8220 Cayman Cat# 138489–18-6 Go-6983 Cayman Cat# 133053–19-7 Ro-31-7549 Cayman Cat# 138516–31-1 Ro-31-8425 Cayman Cat# 145317–11-9 Ro-32-0432 Cayman Cat# 145333–02-4 UCN-1028c Cayman Cat# 121263–19-2 PKC-412 Cayman Cat# 120685–11-2 EEBL Emory ECBDC ( Mo et al., 2019 ) N/A FuGENE Promega Cat# E2311 SBE4-Luc gift from Bert Vogelstein ( Zawel et al., 1998 ) Addgene plasmid # 16495 Dual-Glo luciferase kit Promega Cat# E2920 Oligonucleotides p21-F: 5′-ATGTGTCCTGGTTCCCGTTTC-3′; IDT N/A p21-R: 5′-CATTGTGGGAGGAGCTGTGA-3′; IDT N/A p15-F: 5′- GGACTAGTGGAGAAGGTGCG-3′; IDT N/A p15-R: 5′-GGGCGCTGCCCATCATCATG-3′; IDT N/A GAPDH-F: 5′-GAAGGTGAAGGTCGGAGT-3′; IDT N/A GAPDH-R: 5′-GAAGATGGTGATGGGATTTC-3′.

Techniques:

KEY RESOURCES TABLE

Journal: Cell chemical biology

Article Title: Hypomorph mutation-directed small-molecule protein-protein interaction inducers to restore mutant SMAD4-suppressed TGF-β signaling

doi: 10.1016/j.chembiol.2020.11.010

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Tb cryptate-conjugated mouse monoclonal anti-Flag antibody Cisbio Bioassays Cat# 61FG2TLB D2-conjugated anti-HIS antibody Cisbio Bioassays Cat# 61HISDLF Mouse monoclonal anti-FLAG-HRP antibody Sigma-Aldrich Cat# A8592; RRID: AB_439702 Rabbit polyclonal anti-GST-HRP antibody Sigma-Aldrich Cat# A7340; RRID: AB_258340 Mouse monoclonal anti-β-Actin antibody Sigma-Aldrich Cat# A5441; RRID: AB_476744 Rabbit monoclonal anti-p21 antibody Cell signaling Cat# 2947S; RRID: AB_823586 Mouse monoclonal anti-p15 antibody Santa Cruz Cat# sc-271791; RRID: AB_10709436 SMAD4 (D3M6U) Rabbit mAb Cell signaling Cat# 38454; RRID: AB_2728776 SMAD3 mouse monoclonal Antibody (38-Q) Santa Cruz Cat# sc-101154; RRID: AB_1129525 Mouse Anti-Rabbit IgG (Light-Chain Specific) (D4W3E) mAb (HRP Conjugate) Cell signaling Cat# 93702; RRID: AB_2800208 Peroxidase AffiniPure Goat Anti-Rabbit IgG(H+L) secondary antibody Jackson ImmunoResearch Cat# 111–035-003 Peroxidase AffiniPure Goat Anti-Mouse IgG(H+L) secondary antibody Jackson ImmunoResearch Cat# 115–035-003 Chemicals, Peptides, and Recombinant Proteins Recombinant Human TGF-beta 1 Protein R&D Systems Cat# 240-B Ro-31-8220 Cayman Cat# 138489–18-6 Go-6983 Cayman Cat# 133053–19-7 Ro-31-7549 Cayman Cat# 138516–31-1 Ro-31-8425 Cayman Cat# 145317–11-9 Ro-32-0432 Cayman Cat# 145333–02-4 UCN-1028c Cayman Cat# 121263–19-2 PKC-412 Cayman Cat# 120685–11-2 EEBL Emory ECBDC ( Mo et al., 2019 ) N/A FuGENE Promega Cat# E2311 SBE4-Luc gift from Bert Vogelstein ( Zawel et al., 1998 ) Addgene plasmid # 16495 Dual-Glo luciferase kit Promega Cat# E2920 Oligonucleotides p21-F: 5′-ATGTGTCCTGGTTCCCGTTTC-3′; IDT N/A p21-R: 5′-CATTGTGGGAGGAGCTGTGA-3′; IDT N/A p15-F: 5′- GGACTAGTGGAGAAGGTGCG-3′; IDT N/A p15-R: 5′-GGGCGCTGCCCATCATCATG-3′; IDT N/A GAPDH-F: 5′-GAAGGTGAAGGTCGGAGT-3′; IDT N/A GAPDH-R: 5′-GAAGATGGTGATGGGATTTC-3′.

Techniques: Recombinant, Plasmid Preparation, Luciferase, Viability Assay, Software